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Biochemistry Vitamins Home collection available

Vitamin D3 (25-Hydroxy)

Also known as: 25-Hydroxy Vitamin D

Parameters1 tested
SampleBlood
FastingNot required
Report in24-48 hrs

How to prepare

No fasting needed

You can eat and drink normally before the test.

Good to know

No special preparation needed.

Medicines

Tell our phlebotomist about any medicines you take. Don't stop them without asking your doctor.

Comfortable clothes

Wear a loose sleeve so the sample can be taken easily from your arm.

How it works

Book onlinePick a time slot
Home visitSealed, sterile kit
Lab testingBarcode tracked
ReportIn 24-48 hrs

About this test

This test measures 25-hydroxy vitamin D, the best marker of how much vitamin D your body has stored. Vitamin D helps absorb calcium for strong bones and supports muscles and immunity. Deficiency is very common in India despite plenty of sunshine.

Why is this test done?

  • Check for vitamin D deficiency
  • Find the cause of bone or muscle pain
  • Monitor supplements
  • Bone health check in women and elderly

Symptoms — when should you get tested?

  • Body ache and bone pain
  • Muscle weakness or cramps
  • Tiredness
  • Hair fall
  • Frequent infections
  • Low mood

Normal range

Deficient < 20 ng/mL
Insufficient 20 – 29 ng/mL
Sufficient 30 – 100 ng/mL

Reference ranges differ slightly between labs, methods, age and sex. Always discuss your report with your doctor.

If your result is high

Very high vitamin D (above 100 ng/mL) is usually from taking too many supplements and can raise calcium.

Common reasons
  • Too many high-dose supplements

If your result is low

Low vitamin D weakens bones and muscles and can cause long-lasting body pain.

Common reasons
  • Little time in sunlight
  • Covered clothing, indoor lifestyle
  • Dark skin
  • Obesity
  • Kidney or liver disease

How is the test done?

  1. 1
    Book online

    Choose your slot for home collection or a visit to our centre.

  2. 2
    Blood sample taken

    A trained phlebotomist takes a small blood sample from a vein in your arm with a sealed, single-use needle. It takes about 2 minutes.

  3. 3
    Tested in our lab

    Your barcoded sample is tested on automated machines with strict quality checks.

  4. 4
    Report on WhatsApp

    A pathologist checks the result and the report reaches you on WhatsApp and email.

Save more with a health package

This test is also included in these checkups along with many other tests.

Full Body Checkup – Advanced 14 tests · 31 parameters
Contact for price
Senior Citizen Health Checkup 13 tests · 27 parameters
Contact for price
Women's Wellness Checkup 10 tests · 18 parameters
Contact for price

Frequently asked questions

15–20 minutes of morning sunlight on arms and face, vitamin D-rich foods, and supplements as your doctor prescribes.

No fasting is needed for this test. You can eat and drink normally.

Your report is usually ready in 24-48 hrs after the sample reaches our lab. We send it on WhatsApp and email, and you can also download it from the Reports page.

Please call our team to confirm whether home sample collection is available for your location and test.

This test needs a blood sample. No special preparation needed.

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